Swartz:Protocols/Buffers
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To make own buffers for frequent cloning, these are the recipes:
- Buffer P1 (resuspension):
- 50 mM Tris·Cl, pH 8.0
- 10 mM EDTA
- 100 µg/ml RNase A
- Buffer P2 (Lysis)
- 200 mM NaOH
- 1% SDS (w/v)
- Buffer N3 (Neutralization) – Proprietary but can be determined from Qiagen’s patent
- 4.2 M GuHCl,
- 0.9 M KAc, pH 4.8
- PB (wash/binding buffer):
- 5.0 M GuHCl,
- 30% isopropanol
- PE (wash buffer)
- 50mM Tris, pH 7.5 – Dilute 5X with pure ethanol
- Buffer EB
- 10 mM Tris-Cl, pH 8.5
