Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions

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===Antibiotic Concentrations and Usage===
===Antibiotic Concentrations and Usage===


{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top: 2px solid black" rules="rows" frame="hsides"
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top: 2px solid black" rules="rows" frame="hsides"
|- style="text-align:center; border-top: 1px solid black" valign="top"
|- style="text-align:center; border-top: 1px solid black" valign="top"
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in
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===E.coli Strain & Plasmid Resistance Markers===
===E.coli Strain & Plasmid Resistance Markers===


{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden;" rules="rows" frame="hsides"
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top:2px solid black;" rules="rows" frame="hsides"
|- style="text-align:center; border-top:1px solid black;" valign="top"  
|- style="text-align:center; border-top:1px solid black;" valign="top"  
! style="width:175px;" | E.coli Strain || style="width:150px;" | Plasmid / Cosmid || style="width:150px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts)
! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts)
|- style="text-align:center;"
|- style="text-align:center;"
| BW25113 || pIJ790 (λRED) || None || cat || Replication @ 30C, Loss of λRED @ 37C
| BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30&#176;C, <br/>Loss of λRED @ 37&#176;C
|- style="text-align:center;"
|- style="text-align:center;"
| DH5α || None || N/A || ||
| DH5α || None || N/A || ||
|- style="text-align:center;"
|- style="text-align:center;"
| DH5α || BT340 (FLP Recombinase) || None || cat / blacarb || Initial Replication @ 30C, Induction of FLP Recombinase & loss of plasmid @ 42C, Post recombination growth @37C
| DH5α || BT340 (FLP Recombinase) || None || ''cat'' / ''bla<sub>carb</sub>'' || Initial Replication @ 30&#176;C, <br/>Induction of FLP Recombinase & loss of plasmid @ 42&#176;C, <br/>Post recombination growth @37&#176;C
|- style="text-align:center;"
|- style="text-align:center;"
| ET12567 [dam-13::Tn9] || pUZ8002 || cat || neo ||
| ET12567 [dam-13::Tn9] || pUZ8002 || ''cat'' || ''neo'' ||
|- style="text-align:center;"
|- style="text-align:center;"
| || SuperCos 1 || || neo / blacarb ||  
| || SuperCos 1 || || ''neo'' / ''bla<sub>carb</sub>'' ||  
|- style="text-align:center;"
|- style="text-align:center;"
| || pIJ773 || || acc(3)IV ||
| || pIJ773 || || ''acc(3)IV'' ||
|}
|}


Revision as of 03:53, 27 July 2007

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Antibiotic Concentrations and Usage

Name Stock Concentration (mg/ml) Final Concentration (μg/ml) Usage in 10mL (μL) Dissolve in
Ampicillin 100 100 10 sdH20
Apramycin 100 50 5 sdH20
Chloramphenicol 25 25 10 50%-100% EtOH
Hygromycin 50 25 5 sdH20
Kanamycin 100 50 5 sdH20
Nalidixic Acid 25 25 10 0.2M NaOH
Thiostrepton(e) 50 50 10 100% DMSO or 1:1 DMSO:EtOH
Viomycin 30 30 10 sdH20


E.coli Strain & Plasmid Resistance Markers

E.coli Strain Plasmid / Cosmid E.coli Resistance Marker Plasmid Resistance Marker Temperature Sensitive Replicon (ts)
BW25113 pIJ790 (λRED) None cat Replication @ 30°C,
Loss of λRED @ 37°C
DH5α None N/A
DH5α BT340 (FLP Recombinase) None cat / blacarb Initial Replication @ 30°C,
Induction of FLP Recombinase & loss of plasmid @ 42°C,
Post recombination growth @37°C
ET12567 [dam-13::Tn9] pUZ8002 cat neo
SuperCos 1 neo / blacarb
pIJ773 acc(3)IV



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