Streptomyces:Other Bits/Antibiotic and Resistance Marker Information: Difference between revisions
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{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top: 2px solid black" rules="rows" frame="hsides" | {|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top: 2px solid black" rules="rows" frame="hsides" | ||
|- style="text-align:center; | |- style="text-align:center;" valign="top" | ||
! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ! style="width:175px;" | Name || style="width:150px;" | Stock Concentration (mg/ml) || style="width:150px;"| Final Concentration (μg/ml) || style="width:100px;"| Usage in 10mL (μL) || Dissolve in | ||
|- style="text-align:center;" | |- style="text-align:center; border-top: 1px solid black" | ||
| Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | | Ampicillin || 100 || 100 || 10 || sdH<sub>2</sub>0 | ||
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{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top:2px solid black;" rules="rows" frame="hsides" | {|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top:2px solid black;" rules="rows" frame="hsides" | ||
|- style="text-align:center | |- style="text-align:center;" valign="top" | ||
! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ! style="width:130px;" | E.coli Strain || style="width:120px;" | Plasmid / Cosmid || style="width:120px;"| E.coli Resistance Marker || style="width:100px;"| Plasmid Resistance Marker || Temperature Sensitive Replicon (ts) | ||
|- style="text-align:center;" | |- style="text-align:center; border-top:1px solid black;" | ||
| BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | | BW25113 || pIJ790 (λRED) || None || ''cat'' || Replication @ 30°C, <br/>Loss of λRED @ 37°C | ||
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| || pIJ773 || || ''acc(3)IV'' || | | || pIJ773 || || ''acc(3)IV'' || | ||
|} | |||
===Antibiotic Genes & Information=== | |||
{|cellspacing="5" cellpadding="0" style="width: 770px; border-collapse:collapse; border-top:hidden; border-top:2px solid black;" rules="rows" frame="hsides" | |||
|- style="text-align:center;" valign="top" | |||
! style="width:225px;" | Antibiotic Name || style="width:125px;" | Gene Name || style="width:125px;"| Type || Common Form | |||
|- style="text-align:center; border-top:1px solid black;" | |||
| Ampicillin || ''bla'' || Bactericidal || Sodium | |||
|- style="text-align:center;" | |||
| Apramycin || ''aac(3)IV'' || Aminoglycoside || Sulphate | |||
|- style="text-align:center;" | |||
| Carbenicillin || ''bla<sub>carb</sub>'' || Bactericidal || DiSodium | |||
|- style="text-align:center;" | |||
| Chloramphenicol || ''cat'' || Bacteriostatic || | |||
|- style="text-align:center;" | |||
| Erythromycin || ''ermE'' || Macrolides || | |||
|- style="text-align:center;" | |||
| Hygromycin B || ''hph'' || Aminoglycoside || | |||
|- style="text-align:center;" | |||
| Kanamycin / Neomycin || ''neo'' || Aminoglycoside || MonoSulphate | |||
|- style="text-align:center;" | |||
| Nalidixic Acid || Synthetic || Bacteriostatic || | |||
|- style="text-align:center;" | |||
| Spectinomycin / Streptomycin || ''aadA'' || Aminocyclitol || DiHydrochloride Pentahydrate | |||
|- style="text-align:center;" | |||
| Tetracyclin || ''Tet'' || Aminoglycoside || Hydrochloride | |||
|- style="text-align:center;" | |||
| Thiostrepton || ''tsr'' || Aminoglycoside || | |||
|- style="text-align:center;" | |||
| Viomycin || ''vph'' || Aminoglycoside? || Sulphate | |||
|- style="text-align:center;" | |||
| Vancomycin B || ''vanB'' || Glycopeptide || Hydrochloride | |||
|} | |} | ||
Revision as of 04:11, 27 July 2007
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Antibiotic Concentrations and Usage
E.coli Strain & Plasmid Resistance Markers
Antibiotic Genes & Information
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