Smolke:Protocols/Yeast Media Recipes: Difference between revisions
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**if stored cold or frozen, will crash out of solution; heating helps dissolve | **if stored cold or frozen, will crash out of solution; heating helps dissolve | ||
**detailed useful compound info here: http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/f7129pis.pdf | **detailed useful compound info here: http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/f7129pis.pdf | ||
*For alpha-AAD/LYS2,5 counterselection replace nitrogen source with 2g/L (0.2%) D,L-alpha-aminoadipate | *For alpha-AAD/LYS2,5 counterselection replace nitrogen source with 2g/L (0.2%) D,L-alpha-aminoadipate (Bachem, F-1185) | ||
**dissolve 1 gram alpha-AAD in 20ml of water and pH with KOH to pH 6.0 (otherwise insoluble), filter sterilize and add to YNB with special AA mix | **dissolve 1 gram alpha-AAD in 20ml of water and pH with KOH to pH 6.0 (otherwise insoluble), filter sterilize and add to YNB with special AA mix | ||
**two recipes have been shown to work for this counterselection | **two recipes have been shown to work for this counterselection |
Latest revision as of 14:46, 18 March 2014
Yeast Nitrogen Base Minus Amino Acids (YNB-AA) Recipe
168355)
Autoclave (store at RT for media stocks) To use as working media:
Yeast Nitrogen Base Minus Amino Acids Non-Inducing, Non-Repressing (YNB-AA NINR) Recipe
To use as working media:
Yeast Peptone (YP) Media Recipe
Autoclave (store at RT for media stocks) To use as working media: After autoclaving add:
To pour any of these media stocks as plates:
For yeast KanMX (G418) selection:
For yeast counterselection:
-Michael S. Siddiqui 21:53, 4 January 2011 (EST)* 01/04/2011 (aided by Andrew Babiskin) |