Smolke:Protocols/Yeast Media Recipes: Difference between revisions
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*Cannot use ammonium sulfate as primary nitrogen source | *Cannot use ammonium sulfate as primary nitrogen source | ||
*Add G418 to YP or to YNB with 1 g/L MSG (Sigma G1626) instead of ammounium sulfate | *Add G418 to YP or to YNB with 1 g/L MSG (Sigma G1626) instead of ammounium sulfate | ||
* | *Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months. | ||
* | *Powder G418 sulfate (Geneticin) is stored in chemical room. | ||
*Add 400mg/L for selection (in plates) | *Add 400mg/L for selection (in plates) | ||
*Add 200mg/L for maintenance (in liquid culture media) | *Add 200mg/L for maintenance (in liquid culture media) | ||
-'''[[User:Michael S. Siddiqui|Michael S. Siddiqui]] 21:53, 4 January 2011 (EST)'''* 01/04/2011 (based mainly on communications from [[User:Andrewb|Andrew Babiskin]]) | -'''[[User:Michael S. Siddiqui|Michael S. Siddiqui]] 21:53, 4 January 2011 (EST)'''* 01/04/2011 (based mainly on communications from [[User:Andrewb|Andrew Babiskin]]) |
Revision as of 14:48, 5 August 2013
Yeast Nitrogen Base Minus Amino Acids (YNB-AA) Recipe
168355)
Autoclave (store at RT for media stocks) To use as working media:
Yeast Nitrogen Base Minus Amino Acids Non-Inducing, Non-Repressing (YNB-AA NINR) Recipe
To use as working media:
Yeast Peptone (YP) Media Recipe
Autoclave (store at RT for media stocks) To use as working media: After autoclaving add:
To pour any of these media stocks as plates:
For yeast KanMX (G418) selection:
-Michael S. Siddiqui 21:53, 4 January 2011 (EST)* 01/04/2011 (based mainly on communications from Andrew Babiskin) |