Smolke:Protocols/Yeast Colony PCR: Difference between revisions
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Colony PCR from yeast, either to check inserts etc. or for sequencing. | Colony PCR from yeast, either to check inserts etc. or for sequencing. | ||
==Travis' Version== | ==Travis' Version (Lyticase lysis)== | ||
===Materials=== | ===Materials=== | ||
*Lyticase (from Sigma) | *Lyticase (from Sigma) |
Revision as of 21:40, 26 October 2011
OverviewColony PCR from yeast, either to check inserts etc. or for sequencing. Travis' Version (Lyticase lysis)Materials
ProcedureThe basic idea is breaking the cells with lyticase and heat, then doing PCR.
Notes
Josh's VersionMaterials
Procedure
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