Smolke:Protocols/Sequencing: Difference between revisions
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==Elim's recommended sample format== | ==Elim's recommended sample format== | ||
*At least 50 ng/ul, preferably >100 ng/ul for sample | *At least 50 ng/ul, preferably >100 ng/ul for plasmid sample | ||
*3 uM for primers | *3 uM for primers | ||
*10 ul plasmid and 3 uL primer for each sequencing reaction | *10 ul plasmid and 3 uL primer for each sequencing reaction | ||
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*Use 8-PCR tube strips | *Use 8-PCR tube strips | ||
**I prefer 0.5mL tubes, which give more room to label on the top. -Josh | **I prefer 0.5mL tubes, which give more room to label on the top. -Josh | ||
**Elim biopharm: When possible, please use 8-strip PCR tubes for less than 96 samples and primers, and 96-well plates for equal to or greater than 96 samples | |||
*Tape sample to order form | *Tape sample to order form | ||
**Or recycle the bags that primers come in, and just put the order form in the bag. -Josh | **Or recycle the bags that primers come in, and just put the order form in the bag. -Josh |
Latest revision as of 12:14, 7 July 2012
Elim's recommended sample format
Notes
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