Smolke:Protocols/Quick yeast transformation: Difference between revisions
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(New page: {{Smolke_Top}} ==Overview== Adapted from Gietz, Nature Protocols 2007 (dx.doiorg/10.1038/nprot.2007.14), this works similarly to the standard, longer method. -Leo ==Materials== *YPD *Ste...) |
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#Grow an overnight culture of your chosen yeast strain in appropriate media. | #Grow an overnight culture of your chosen yeast strain in appropriate media. | ||
#*Alternatively, you can grow serial dilutions of your chosen strain, and one of them will likely be at the appropriate OD the next morning. For me, picking some cells into 3 mL of media, then taking 300 ul of this into a 25 mL flask works pretty well. -Leo | #*Alternatively, you can grow serial dilutions of your chosen strain, and one of them will likely be at the appropriate OD the next morning. For me, picking some cells into 3 mL of media, then taking 300 ul of this into a 25 mL flask works pretty well. -Leo | ||
#*Or you can pick ~10ul plated culture per transformation and resuspend in water. Ideally ~1e8 cells/transformation (can check OD). More variable but generally works. -Leo | |||
===Day 1=== | ===Day 1=== |
Revision as of 10:20, 19 June 2013
OverviewAdapted from Gietz, Nature Protocols 2007 (dx.doiorg/10.1038/nprot.2007.14), this works similarly to the standard, longer method. -Leo Materials
ProcedureDay 0
Day 1
Day 3Colonies should be ready. Some strains/plasmids may take an extra day. Notes |