Smolke:Protocols/Feeding yeast cultures: Difference between revisions
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**Storage: 4C | **Storage: 4C | ||
====Growing and Feeding Cultures=== | ====Growing and Feeding Cultures==== | ||
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*Set up an overnight culture of strains in selective media (3mL). (Use synthetic complete in place of YPD) | *Set up an overnight culture of strains in selective media (3mL). (Use synthetic complete in place of YPD) |
Revision as of 20:12, 11 January 2012
OverviewThis protocol is used to set up cultures, feed substrates, and harvest metabolites from media for analysis by LCMS. ProcedureMaterials
MethodMaking Substrate Stocks
Growing and Feeding CulturesDay 1:
Day 2:
Analysis
ReferencesContact |