Smolke:Lab Jobs

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==Laboratory Jobs and Administrative Assignments==
==Laboratory Jobs and Administrative Assignments==
-
'''Andy'''           
+
'''Ben'''           
-
:plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing)
+
:-T plates (II), -U plates (II)
-
:''Biacore''
+
:''4 refrigerator/cold room organization''
 +
 
 +
'''Calvin'''
 +
:10x sugar stocks (20% dextrose), 60% glycerol
 +
:''''
'''Christina'''
'''Christina'''
:''general lab ordering''
:''general lab ordering''
-
 
-
'''Drew'''
 
-
: electrocompetent cells (I), water baths (shared with Endy lab)
 
-
:''4 refrigerator/cold room organization; freezer (-80/-20)''
 
'''Julie'''
'''Julie'''
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'''Isis'''
'''Isis'''
-
:LB+amp plates (II)
+
:LB+amp plates (III)
-
:''software, secondary lab safety officer''
+
:''journal club, secondary lab safety officer''
-
 
+
-
'''Jay'''
+
-
:10x sugar stocks (20% dextrose), 60% glycerol
+
-
:''CE, cryogenic storage LN2 refill (I)''
+
'''Joy'''
'''Joy'''
-
:LB+amp plates (III)
+
:LB+amp plates (II)
-
:''computer administrator and databases (back up)''
+
:''computer administrator and databases, including Lab/SoE server (back up)''
'''Kate'''
'''Kate'''
Line 35: Line 31:
'''Kathy'''
'''Kathy'''
-
:electrocompetent cells (II), general cleaning of high speed centrifuge
+
:competent cells (II), general cleaning of high speed centrifuge
-
:''Quanta, MACSQuant''
+
:''Quanta, MACSQuant, Filemaker database''
'''Maureen'''
'''Maureen'''
:sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II)
:sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II)
-
:''radioactivity safety officer''
+
:''radioactivity safety officer, Biacore''
'''Melina'''
'''Melina'''
:Gibson master mix, antibiotics
:Gibson master mix, antibiotics
-
:''lab wiki, TC Room (II)''
+
:''lab wiki, TC Room (CO2 cylinders)''
-
'''Michael'''
+
'''Mike S'''
-
:-T plates, YPD plates, YPD [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]]
+
:-T plates (I), YPD plates, YPD [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]]
-
:''LC-MS''
+
:''LC-IT''
 +
 
 +
'''Michael R'''
 +
:LB+amp plates (I)
 +
:''cryogenic storage LN2 refill (I)''
 +
 
 +
'''Peter'''
 +
:LB, pH meters, aliquot dNTPs, water baths (shared with Endy lab)
 +
:''TC Room (internal); cryogenic storage LN2 refill (II)''
'''Remus'''
'''Remus'''
Line 54: Line 58:
:''general enzyme ordering''
:''general enzyme ordering''
-
'''Ryan'''
+
'''Shireen'''
-
:LB+amp plates (II)
+
:competent cells (I)
-
:''TC Room; cryogenic storage LN2 refill (II)''
+
:''freezer (-80/-20)''
'''Stephanie'''
'''Stephanie'''
:amino acid stocks, 10x drop out stocks for plates (I)
:amino acid stocks, 10x drop out stocks for plates (I)
-
:''LC and fraction collector, inventory chemical waste bottles/containers (fume hood)''
+
:''LC-QQQ, inventory chemical waste bottles/containers (fume hood)''
'''Yanran'''
'''Yanran'''
-
:LB (I), pH meters, aliquot dNTPs
+
:YNB/NINR [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]], SOC
-
:''journal club''
+
:''semiprep-LC''
-
'''Yen-Hsiang'''           
+
'''Yen-Hsiang (returns 9/15)'''           
-
:YNB [[http://openwetware.org/wiki/Smolke:Protocols/Yeast_Media_Recipes]], NINR, SOC
+
:plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing)
-
:''computer administrator and databases''
+
:''computer administrator and databases; lab/SoE server''

Revision as of 20:39, 15 August 2014

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Laboratory Jobs and Administrative Assignments

Ben

-T plates (II), -U plates (II)
4 refrigerator/cold room organization

Calvin

10x sugar stocks (20% dextrose), 60% glycerol
'

Christina

general lab ordering

Julie

glass beads, toothpicks, used plate sterilization, glass/plastic washing sterilization, disposable sterilization
safety officer, general lab organization/maintenance, sharps, cleaning general areas, order inventory, inventory MSDS sheets, organize library

Isis

LB+amp plates (III)
journal club, secondary lab safety officer

Joy

LB+amp plates (II)
computer administrator and databases, including Lab/SoE server (back up)

Kate

-U plates, -UT plates (I)
schedule II officer, general enzyme ordering and information (back up)

Kathy

competent cells (II), general cleaning of high speed centrifuge
Quanta, MACSQuant, Filemaker database

Maureen

sodium acetate, TAE stocks, 10x drop out stocks for plates (II), -UT plates (II)
radioactivity safety officer, Biacore

Melina

Gibson master mix, antibiotics
lab wiki, TC Room (CO2 cylinders)

Mike S

-T plates (I), YPD plates, YPD [[1]]
LC-IT

Michael R

LB+amp plates (I)
cryogenic storage LN2 refill (I)

Peter

LB, pH meters, aliquot dNTPs, water baths (shared with Endy lab)
TC Room (internal); cryogenic storage LN2 refill (II)

Remus

sterile water, EDTA stocks, TC room water bath
general enzyme ordering

Shireen

competent cells (I)
freezer (-80/-20)

Stephanie

amino acid stocks, 10x drop out stocks for plates (I)
LC-QQQ, inventory chemical waste bottles/containers (fume hood)

Yanran

YNB/NINR [[2]], SOC
semiprep-LC

Yen-Hsiang (returns 9/15)

plasmid prep buffers (P1, P2, N3, PE, EB, stocks - confirm with testing)
computer administrator and databases; lab/SoE server


Notes

  • Please make sure BEFORE you leave for a vacation that you check on stocks (or whatever is appropriate) for your lab job. Make sure you anticipate usage over the time you will be out of the lab and that there will be enough stocks, etc to last the lab before you return. If you are concerned about lab usage while you are gone, it is your responsibility to ask someone in the lab to monitor it for you and cover while you are away.
  • General lab courtesy - if you use a whole sleeve of plates, make another. If you use a lot of a stock or finish it, make some more.
  • Everyone should help aliquot electrocomp cells.
  • Solution I and Solution II are now the responsibility of those who use them regularly. TBE is also the responsibility of those who are using it regularly.

How It Works

Each person is assigned a laboratory job (or set of jobs based on intensity of the assigned job) and an administrative role (indicated in italics). Please keep up with your assigned jobs. Note, that it is everyone's responsibility to load tips, load the dishwasher, and rinse out your glassware. Also, while general DO plates are made (-U, -T, and -UT) as common stocks, all other DO plates are made by the individual using them.

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