Smolke:Lab Jobs

Laboratory Jobs and Administrative Assignments

Andy

plasmid prep buffers (P1, P2, N3, PE, EB, stocks), aliquot dNTPs (II)

Biacore

Christina

general lab ordering

Drew

electrocompetent cells (I), water baths

4 refrigerator/cold room organization; freezer (-80/-20)

Julie

glass beads, toothpicks, used plate sterilization, glass/plastic washing sterilization, disposable sterilization

safety officer, general lab organization/maintenance, sharps, cleaning general areas, order inventory, inventory MSDS sheets, organize library

Isis

LB plates (II)

databases and software, secondary lab safety officer

Jay

10x sugar stocks (dextrose), TAE stocks, 60% glycerol, sodium acetate

CE, cryogenic storage LN2 refill (I)

Joe

amino acid stocks, 10x drop out stocks for plates (I)

general enzyme ordering and information

Josh M

off-site

Josh W

LB (I), pH meters, aliquot dNTPs (I)

journal club, CE (back up)

Kate

-U plates, -UT plates (I)

general enzyme ordering and information (back up)

Kathy

electrocompetent cells (II), general cleaning of high speed centrifuge

Quanta, MACSQuant

Leo

LB plates (I)

radioactivity safety officer

Melina

LB (II), antibiotics

lab wiki

Michael

-T plates, YPD plates, YPD [[1]]

LC-MS

Remus

sterile water, EDTA stocks, TC room water bath

general enzyme ordering, TC Room (II)

Ryan

LB plates (III)

TC Room; cryogenic storage LN2 refill (II)

Stephanie

amino acid stocks, 10x drop out stocks for plates (I)

LC and fraction collector, inventory chemical waste bottles/containers (fume hood)

Yen-Hsiang

YNB [[2]], NINR, -UT plates (II), SOC

computer administrator and databases

Notes

• Please make sure BEFORE you leave for a vacation that you check on stocks (or whatever is appropriate) for your lab job. Make sure you anticipate usage over the time you will be out of the lab and that there will be enough stocks, etc to last the lab before you return. If you are concerned about lab usage while you are gone, it is your responsibility to ask someone in the lab to monitor it for you and cover while you are away.
• General lab courtesy - if you use a whole sleeve of plates, make another. If you use a lot of a stock or finish it, make some more.
• Everyone should help aliquot electrocomp cells.
• Solution I and Solution II are now the responsibility of those who use them regularly. TBE is also the responsibility of those who are using it regularly.

How It Works

Each person is assigned a laboratory job (or set of jobs based on intensity of the assigned job) and an administrative role (indicated in italics). Please keep up with your assigned jobs. Note, that it is everyone's responsibility to load tips, load the dishwasher, and rinse out your glassware. Also, while general DO plates are made (-U, -T, and -UT) as common stocks, all other DO plates are made by the individual using them.