Sean Clarke/109 notes: Difference between revisions
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[[20.109:Module_1]] | [[20.109:Module_1]] | ||
==M13 genome== | ==M13 genome== | ||
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| || translational restart product of g1, | | || translational restart product of g1, | ||
|} | |} | ||
==oligos== | |||
NO150/151 | |||
g8p myc' Top / Bottom | |||
CTC GAA CAG AAG CTT ATC TCT GAA GAA GAC CTT GCA | |||
ACG TGA GCT TGT CTT CGA ATA GAG ACT TCT TCT GGA | |||
NO154/155 | |||
g3p myc' Top / Bottom | |||
Revision as of 12:30, 8 February 2007
M13 genome
6407 bp single-stranded circular DNA
filamentous, male-specific? coliphage related to fd and f1
- p3,p8,p9 = virion structural proteins, p3 and p8 have N-term signal peptides that are hydrophobic
11 coding genes: 5 on surface, 6 for phage maturation in host
| position | notes | size (aa) | MW | |
|---|---|---|---|---|
| III | 1579-2853(1?) | capsid protein A, precursor has 18 extra aa, coding seq starts at 1633 | 424/408 | 42675 |
| VIII | 1301-1525 | encodes precursor w/23 extra N-term aa, pVIII is major virion capsid protein B | 75/50 | |
| IX | 1206-1304(2?) | overlaps g8 by 1 nt, small virion capsid protein (C-protein) | 32 | |
| VI | 2856-3194 | small hydrophobic part of the capsid with p3 | 112 | 12264 |
| V | 843-1106 | helix-destabilizing protein in ss viral DNA synthesis | ||
| II | 6006-831 | req'd for dsDNA replication | 410 | 46,117 |
| X | 496-831 | initiated in-frame w/in g2, same as p2 C-term | 12,670 | |
| VII | 1108-1209 | capsid companion to p9 | 33 | |
| I | 3196-4242 | phage assembly | 348 | 35000 in vitro, 39500 predicted |
| IV | 4220-5500 | phage assembly, barrel in mb, 23 nt overlap with C-term of gI, RBS 4204-27 | 426 | 45791 |
| XI | translational restart product of g1, |
oligos
NO150/151
g8p myc' Top / Bottom
CTC GAA CAG AAG CTT ATC TCT GAA GAA GAC CTT GCA
ACG TGA GCT TGT CTT CGA ATA GAG ACT TCT TCT GGA
NO154/155 g3p myc' Top / Bottom
