Sean Clarke/109 notes

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20.109:Module_1


M13 genome

6407 bp single-stranded circular DNA

filamentous, male-specific? coliphage related to fd and f1

  • p3,p8,p9 = virion structural proteins, p3 and p8 have N-term signal peptides that are hydrophobic

11 coding genes: 5 on surface, 6 for phage maturation in host

position notes size (aa) MW
III 1579-2853(1?) capsid protein A, precursor has 18 extra aa, coding seq starts at 1633 424/408 42675
VIII 1301-1525 encodes precursor w/23 extra N-term aa, pVIII is major virion capsid protein B 75/50
IX 1206-1304(2?) overlaps g8 by 1 nt, small virion capsid protein (C-protein) 32
VI 2856-3194 small hydrophobic part of the capsid with p3 112 12264
V 843-1106 helix-destabilizing protein in ss viral DNA synthesis
II 6006-831 req'd for dsDNA replication 410 46,117
X 496-831 initiated in-frame w/in g2, same as p2 C-term 12,670
VII 1108-1209 capsid companion to p9 33
I 3196-4242 phage assembly 348 35000 in vitro, 39500 predicted
IV 4220-5500 phage assembly, barrel in mb, 23 nt overlap with C-term of gI, RBS 4204-27 426 45791
XI translational restart product of g1,

oligos

NO150/151

g8p myc' Top / Bottom

   CTC GAA CAG AAG CTT ATC TCT GAA GAA GAC CTT GCA

ACG TGA GCT TGT CTT CGA ATA GAG ACT TCT TCT GGA

NO154/155

g3p myc' Top / Bottom

GAT CTA GAA CAG AAG CTT ATC TCT GAA GAA CTA

    ATC TTG TCT TCG AAT AGA GAC TTC TTC TGG ATC TAG
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