Sack and Yarov-Yarovoy:Protocols

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==Flourophore Labeling==
==Flourophore Labeling==
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[[Sack: GxTx Purification|GxTx Purification 11/22/11]]<br>  
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[[Sack: GxTx Purification|GxTx Purification 11/22/11]][http://openwetware.org/images/9/92/112211_OMIXC18_GxTX_purification.docx  .]<br>  
[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]]<br>
[[Sack: Alyknefluor Azide Beads|Alyknefluor Azide Beads 11/11/11]]<br>
[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]]<br>
[[Sack: Maleimide Labeling| Maleimide Labeling 11/08/11]]<br>

Revision as of 17:46, 10 October 2012

Cell Culture

Cell Media with Selection Agents (Blasticidin, Zeocin, Geneticin) 04/05/12 .
Cell Media with Selection Agents (Blasticidin, Zeocin) 11/15/11 .
Cell Splitting Protocol 03/02/12.
Cell Transfection (Lipofectamine LTX) 05/01/12.
Cell Transfection (Lipofectamine) 08/14/12.
Cell Transfection (Novachoice) 05/04/12.
Cell Harvesting for Electrophysiology 07/18/12.
Cell Plating for Electrophysiology 10/21/11 .
Freezing CHO Cells 04/05/12.
Plating Subclones for Ephys 09/29/11.
Plating Subclones for Flourescent Screening 04/05/12.
Subcloning Cells 02/23/11.
Testing TREX Subclones Ephys 04/06/11.
Thawing CHO cells 07/09/12.

Flourophore Labeling

GxTx Purification 11/22/11.
Alyknefluor Azide Beads 11/11/11
Maleimide Labeling 11/08/11
Maleimide Labeling 10/08/12




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