SSB13Ntbk - Apr16: Difference between revisions
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(New page: ==Golden Gate Assembly== 3.5 μl ddH2O 1 μl Ligase Buffer .5 μl Ligase .5 μl BsmB1 1 μl per construct (4 total) .5 μl vector) |
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==Golden Gate Assembly== | ==Golden Gate Assembly== | ||
3.5 μl ddH2O | 3.5 μl ddH2O <br> | ||
1 μl Ligase Buffer | 1 μl Ligase Buffer <br> | ||
.5 μl Ligase | .5 μl Ligase <br> | ||
.5 μl BsmB1 | .5 μl BsmB1 <br> | ||
1 μl per construct (4 total) | 1 μl per construct (4 total) <br> | ||
.5 μl vector | .5 μl vector <br> | ||
Latest revision as of 10:21, 16 April 2013
Golden Gate Assembly
3.5 μl ddH2O
1 μl Ligase Buffer
.5 μl Ligase
.5 μl BsmB1
1 μl per construct (4 total)
.5 μl vector
