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- 13:21, 16 April 2013 (hist) (diff) SSB13Ntbk - Apr16 (→Golden Gate Assembly) (top)
- 13:20, 16 April 2013 (hist) (diff) N SSB13Ntbk - Apr16 (New page: ==Golden Gate Assembly== 3.5 μl ddH2O 1 μl Ligase Buffer .5 μl Ligase .5 μl BsmB1 1 μl per construct (4 total) .5 μl vector)
- 13:18, 16 April 2013 (hist) (diff) SBB13Ntbk-ChristinaS (top)
- 18:00, 2 April 2013 (hist) (diff) N SSB13Ntbk - Apr2 (New page: Got colonies from the transformation performed two weeks ago. Class redid their restriction digest and we all ran our saved samples on the gel. I saw my digest.) (top)
- 17:59, 2 April 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 13:56, 21 March 2013 (hist) (diff) SSB13Ntbk - Mar21 (top)
- 12:57, 21 March 2013 (hist) (diff) N SSB13Ntbk - Mar21 (New page: ===Ligation of EcoRI/BamHI digests=== *Set up the following reaction: 6.5uL ddH2O 1uL T4 DNA Ligase Buffer (small red or black-striped tubes) 1uL Vector digest 1uL Insert digest ...)
- 12:39, 21 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 03:09, 21 March 2013 (hist) (diff) N SSB13Ntbk - Mar19 (New page: ===Regular Zymo Cleanup=== #Add 180 uL of Zymo ADB buffer to PCR reaction. #Transfer into the Zymo column #spin through, discard waste. #Add 200 uL of Zymo Wash Buffer #spin through, disc...) (top)
- 03:04, 21 March 2013 (hist) (diff) SBB13Ntbk - Mar14 (top)
- 03:03, 21 March 2013 (hist) (diff) N SBB13Ntbk - Mar14 (New page: The PCA amplification did not yield enough PCR product, so need to do re-amplification. #Ran an analytical gel, 1%, 10ul of PCR product. Thankfully saw PCR product, but a very small amoun...)
- 02:58, 21 March 2013 (hist) (diff) SBB13Ntbk - Mar12 (top)
- 02:55, 21 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 02:55, 21 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 02:53, 21 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 13:03, 12 March 2013 (hist) (diff) N SBB13Ntbk - Mar12 (New page: ==Regular Zymo Cleanup== #Add 180 uL of Zymo ADB buffer (brown bottle) to a 33uL or 50uL reaction. #Transfer into the Zymo column (small clear guys) #spin through (top speed, 30 sec), dis...)
- 12:52, 12 March 2013 (hist) (diff) SBB13Ntbk - Mar8 (top)
- 12:52, 12 March 2013 (hist) (diff) SBB13Ntbk - Mar8
- 12:51, 12 March 2013 (hist) (diff) N SBB13Ntbk - Mar8 (New page: ==PCA Assembly - Part 1==<br> PCA mixture 38 uL ddH2O 5 ul 10x expand buffer 5 ul 2mM dNTPs 1 ul oligo mixture (100mM) .75 Expand polymerase gave the mix to Anderson to run PCA)
- 12:47, 12 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 12:46, 12 March 2013 (hist) (diff) N SBB13Ntbk - Mar7 (New page: <pre> PCA quiC-4 - oligo1-16 (464bp, pca1) PCR pca1 with quiC-NewForward/Reverse (418bp, pca2) Digest pca2 (EcoRI/BglII, 402+10+6,L,...) (top)
- 12:45, 12 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS (Replacing page with ' March 7, 2013 <br>')
- 12:45, 12 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 12:43, 12 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 16:29, 5 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 16:23, 5 March 2013 (hist) (diff) SBB13Ntbk-ChristinaS
- 15:49, 5 March 2013 (hist) (diff) Sbb13-OligoWishList
- 15:48, 5 March 2013 (hist) (diff) Sbb13-OligoWishList
- 15:46, 5 March 2013 (hist) (diff) N SBB13Ntbk-ChristinaS (New page: PCA quiC-4)
- 15:37, 5 March 2013 (hist) (diff) SynBioBootcamp:Notebook/SynBioBootcamp13 (→Individual Notebooks)
- 00:59, 6 February 2013 (hist) (diff) User:Christina Schlesinger (top)
- 00:56, 6 February 2013 (hist) (diff) SBB13 project (→JCAnderson 13:43, 5 February 2013 (EST) Targets for further analysis:)
- 18:51, 5 February 2013 (hist) (diff) N User talk:Christina Schlesinger (top)
- 18:51, 5 February 2013 (hist) (diff) N User:Christina Schlesinger
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