SBB09 Construct32: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 10: | Line 10: | ||
OWRL-024-R Reverse biobricking of Intimin EaeA | OWRL-024-R Reverse biobricking of Intimin EaeA | ||
GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC | GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC | ||
Revision as of 13:34, 9 February 2009
Biobricking of Intimin EaeA
PCR OWRL-023-F/OWRL-024-R on {<Intimin EaeA>} (1887bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 910+3039,L)
Product is pBca9495CA-M10032
---
OWRL-023-F Forward biobricking of Intimin EaeA
GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG
OWRL-024-R Reverse biobricking of Intimin EaeA
GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC