SBB09 Construct32

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Biobricking of Intimin EaeA 

PCR OWRL-023-F/OWRL-024-R on {<Intimin EaeA>} 	(1887bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 				(EcoRI/BamHI, 910+3039,L)
Product is pBca9495CA-M10032
---
OWRL-023-F	Forward biobricking of Intimin EaeA
GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG
OWRL-024-R	Reverse biobricking of Intimin EaeA
GCAAAggatccGCCTTGGTTTGATCAAAAAATATAACCGCAC
 JCA:  Reverse oligo can be reused.  See revision:
Biobricking of Intimin EaeA 

PCR OWRL-023-F/Bhs003R	 on {<Intimin EaeA>} 	(1888 bp, EcoRI/BamHI)
Sub into pBca9495CA-Bca1144#5 				(EcoRI/BamHI, 910+3039,L)
Product is pBca9495CA-M10032  {<eaeA refactored>}
---
OWRL-023-F	Forward biobricking of Intimin EaeA
GCGTAgaattcATGagatctAATGGTGAAAATTATTTTAAATTGGGTTCGG
Bhs003R	Reverse BamHI for a~eaeA_Display>	
GCAAAggatccGGCCTTGGTTTGATCAAAAAATATAACCGCAC
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