Registry/Measurement kit/Notebook/2007-7-8: Difference between revisions
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(New page: ==M/N Digest of I2055== *Hopefully it has the promoter... *Should ligate, transform, sequence. ==Colony PCRs== *Running a gel) |
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==M/N Digest of I2055== | ==M/N Digest of I2055== | ||
*Hopefully it has the promoter | *Hopefully it has the promoter | ||
* | *Ligated into 1AK3 and transformed. | ||
==Colony PCRs== | ==Colony PCRs== | ||
*Running a gel | *Running a gel | ||
[[Image:07.07.08_-_colony.jpg|thumb|left|Gel from 7/8/07]] | |||
**loading order:T9002 in 3K3 (4-1), I2055 in 1AK3 (1-4), E0240 in 3K3(4-1), E0240 in 1AK3(1,2). | |||
*Seems to be a problem with 3K3 (perhaps not cut?) | |||
*Making cultures to sequence: | |||
**T9002-3K3(bad): #3 | |||
**I2055-1AK3: #1 | |||
**E0240-3K#(bad): #3 | |||
**E0240-1AK3: #1 | |||
*Since 3K3 may be causing the problems, making o/n of it to mini-prep tomorrow and digest. | |||
==I2056== | |||
*Prepared sequencing reaction. | |||
Revision as of 06:55, 9 July 2007
M/N Digest of I2055
- Hopefully it has the promoter
- Ligated into 1AK3 and transformed.
Colony PCRs
- Running a gel
- loading order:T9002 in 3K3 (4-1), I2055 in 1AK3 (1-4), E0240 in 3K3(4-1), E0240 in 1AK3(1,2).
- Seems to be a problem with 3K3 (perhaps not cut?)
- Making cultures to sequence:
- T9002-3K3(bad): #3
- I2055-1AK3: #1
- E0240-3K#(bad): #3
- E0240-1AK3: #1
- Since 3K3 may be causing the problems, making o/n of it to mini-prep tomorrow and digest.
I2056
- Prepared sequencing reaction.
