Registry/Measurement kit/Notebook/2007-7-5: Difference between revisions
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==E0240== | ==E0240== | ||
*Primers arrived to insert BB sites. See [[Registry/Measurement kit/Notebook/2007-7-2|7/2.]] | *Primers arrived to insert BB sites. See [[Registry/Measurement kit/Notebook/2007-7-2#Primer_Design|7/2.]] | ||
*Diluting primers, starting PCR on E0240. Tm = 53.5 | *Diluting primers, starting PCR on E0240. Tm = 53.5 | ||
Latest revision as of 14:37, 5 July 2007
I2056
- running 10uL of PCR on a small gel
I2055
- Sent samples to be sequenced
T9002
- Did PCR purification on MfeI digest, but DNA is very dilute: 13.1 ng/uL
- Trying double digest in parallel with NsiI digest
E0240
- Primers arrived to insert BB sites. See 7/2.
- Diluting primers, starting PCR on E0240. Tm = 53.5
