QRT-PCR/Two tubes: Difference between revisions
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(New page: The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of...) |
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Revision as of 13:12, 9 October 2007
The first tube produces cDNA from an RNA sample. The cDNA can either be produced using a single sequence-specific reverse transcription primer designed to anneal only to the transcript of interest or using reverse transcription primers that anneal to all RNA molecules.
The second tube is sequence specific qPCR step.
