QRT-PCR/Single tube

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(Real-time RT-PCR moved to Quantitative reverse transcriptase PCR: naming here is difficult but I think RT RT PCR is confusing; real-time doesn't tell you much; Q tells you that this is all about quantification;)
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#REDIRECT [[Quantitative reverse transcriptase PCR]]
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This protocol describes one-step real time quantitivie PCR to quantify relative levels of RNA in
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== Starting Materials ==
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* Validated PCR primers that are efficient over the range of RNA that you are assaying. 
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*
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== Basic Principle ==
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* RNA is reverse transcribed in a single well. 
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* PCR reaction procedes.  The levels of template are quantified during each cycle. 
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== Protocol ==
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== Analysis ==
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Delta-Delta Ct Method.  See Livak KJ, Schmittgen.  Methods '''25''' 402-408 (2001)

Revision as of 15:10, 26 March 2007

This protocol describes one-step real time quantitivie PCR to quantify relative levels of RNA in

Contents

Starting Materials

  • Validated PCR primers that are efficient over the range of RNA that you are assaying.

Basic Principle

  • RNA is reverse transcribed in a single well.
  • PCR reaction procedes. The levels of template are quantified during each cycle.

Protocol

Analysis

Delta-Delta Ct Method. See Livak KJ, Schmittgen. Methods 25 402-408 (2001)

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