QRT-PCR: Difference between revisions
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This page focuses on information specific to quantitative reverse transcriptase PCR rather than quantitative PCR in general. | This page focuses on information specific to quantitative reverse transcriptase PCR rather than quantitative PCR in general. | ||
==Notes== | |||
*The most commonly used specialist reverse transcriptase enzyme for cDNA production is AMV reverse transcriptase. It has RNase H activity (so that RNA molecules are only transcribed once) and has a high temperature stability (to reduce RNA secondary structure and nonspecific primer annealing). | |||
==Protocols== | |||
*[[qRT-PCR/Two tube]] | |||
*[[qRT-PCR/Single tube]] | |||
==References== | |||
<biblio> | |||
#MeasuringGeneExpression isbn=0415374723 | |||
</biblio> | |||
[[Category:Protocol]] [[Category:In vitro]] [[Category:DNA]] [[Category:RNA]] | [[Category:Protocol]] [[Category:In vitro]] [[Category:DNA]] [[Category:RNA]] | ||
Revision as of 13:08, 9 October 2007
See Q-PCR for much useful information.
This page focuses on information specific to quantitative reverse transcriptase PCR rather than quantitative PCR in general.
Notes
- The most commonly used specialist reverse transcriptase enzyme for cDNA production is AMV reverse transcriptase. It has RNase H activity (so that RNA molecules are only transcribed once) and has a high temperature stability (to reduce RNA secondary structure and nonspecific primer annealing).
