PMSF

Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.

Molecular W.: 174.19

Procurement

You can buy PMSF for example at Pierce or Sigma.

Use

PMSF is not soluble in water, dissolve in ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. A common final concentration of PMSF is 1mM of course depending on the experimental setup.

Safety

PMSF is poisonous and should be handled carefully.