PMSF: Difference between revisions
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==Purpose== | ==Purpose== | ||
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an inhibitor of protease activity for example in protein purification or co-immunoprecipitation. | PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation. | ||
Molecular W.: 174.19 | Molecular W.: 174.19 | ||
Revision as of 15:59, 8 January 2011
Purpose
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.
Molecular W.: 174.19
Procurement
You can buy PMSF for example at Pierce or Sigma.
Use
PMSF is not soluble in water, dissolve in ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C. A common final concentration of PMSF is 1mM of course depending on the experimental setup.
Safety
PMSF is poisonous and should be handled carefully.
