PMSF

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Purpose)
Current revision (19:04, 8 January 2011) (view source)
(Purpose)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
==Purpose==
==Purpose==
-
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation.
+
PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation. It has no effect on other proteases that might be present.  It can also inactivate some non-proteolytic enzymes that contain serine in the active site.
Molecular W.: 174.19
Molecular W.: 174.19
Line 8: Line 8:
==Use==
==Use==
-
PMSF is not soluble in water, dissolve in ethanol or methanol.
+
PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol.
-
200 mM stock solution (0.035 g/mL) is stable for months at 4°C.
+
200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better.
-
A common final concentration of PMSF is 1mM of course depending on the experimental setup.
+
A common final concentration of PMSF is 1mM.  PMSF is extremely toxic, and preparation of solutions should be done very carefully.  PMSF is not stable in alkaline conditions, or in water.  For disposal, add alkali in water and wait to dispose.
==Safety==
==Safety==
-
PMSF is poisonous and should be handled carefully.
+
PMSF is extremely poisonous and should be handled carefully.
[[Category:Material]] [[Category:Chemical]]
[[Category:Material]] [[Category:Chemical]]

Current revision

Contents

Purpose

PMSF stands for Phenylmethylsulfonyl fluoride. It is used as an irreversible inhibitor of serine protease activity for example in protein purification or co-immunoprecipitation. It has no effect on other proteases that might be present. It can also inactivate some non-proteolytic enzymes that contain serine in the active site.

Molecular W.: 174.19

Procurement

You can buy PMSF for example at Pierce or Sigma.

Use

PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM. PMSF is extremely toxic, and preparation of solutions should be done very carefully. PMSF is not stable in alkaline conditions, or in water. For disposal, add alkali in water and wait to dispose.

Safety

PMSF is extremely poisonous and should be handled carefully.

Personal tools