OhioMod2013:Methods/Liposomes: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: Got liposomes from Bryant Yung of Dr. Lee's lab. Two tubes of: *QTCN: Quaternary-Tertiary Lipid Amine Cationic Nanoparticles (1 mg/mL) *SPLN: Small Peptide Lipid Nanoparticles (2 mg/mL) Ke...) |
|||
| Line 6: | Line 6: | ||
==Combine with DNA origami== | ==Combine with DNA origami== | ||
Take lipid stock, combine with oligo at ratio of 1:15 DNA/lipid(weight/weight) in 10x transfection medium. | *Take lipid stock, combine with oligo at ratio of 1:15 DNA/lipid(weight/weight) in 10x transfection medium. | ||
Set aside 10-15 min to allow for electrostatic interaction. | *Set aside 10-15 min to allow for electrostatic interaction. | ||
Dilute to final volume | *Dilute to final volume | ||
Extrude through polycarbonate filter, or other sterile filter in general | *Extrude through polycarbonate filter, or other sterile filter in general | ||
Revision as of 06:47, 2 July 2013
Got liposomes from Bryant Yung of Dr. Lee's lab. Two tubes of:
- QTCN: Quaternary-Tertiary Lipid Amine Cationic Nanoparticles (1 mg/mL)
- SPLN: Small Peptide Lipid Nanoparticles (2 mg/mL)
Kept in 40% ethanol in 10 mM citric acid buffer (pH of 5). The lipid mixture is composed of DODAP, Lac-DOPE, DOPE, DMG-PEG, and with or without gramacidin A, at a molar ratio of 50:10:28:2:10 respectively.
Combine with DNA origami
- Take lipid stock, combine with oligo at ratio of 1:15 DNA/lipid(weight/weight) in 10x transfection medium.
- Set aside 10-15 min to allow for electrostatic interaction.
- Dilute to final volume
- Extrude through polycarbonate filter, or other sterile filter in general
