Neomycin
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==Experimental== | ==Experimental== | ||
| + | |||
| + | ===Target organism=== | ||
| + | bacteria, mammalian cells | ||
===Stock solution=== | ===Stock solution=== | ||
| + | 10mg/ml in water | ||
| + | |||
===Working solution=== | ===Working solution=== | ||
| + | 10-50µg/ml | ||
| + | |||
| + | ==Description== | ||
| + | |||
| + | ===Mode of action=== | ||
| + | Binds to ribosomal components and inhibits protein synthesis. | ||
| + | |||
| + | ===Mode of resistance=== | ||
| + | ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | ||
| + | |||
| + | ==Related antibiotics== | ||
| + | [[kanamycin]], [[G418]] | ||
| - | == | + | ==External links== |
| + | * list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910] | ||
| + | * list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | ||
| + | * forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | ||
Revision as of 09:02, 4 August 2006
Contents |
Experimental
Target organism
bacteria, mammalian cells
Stock solution
10mg/ml in water
Working solution
10-50µg/ml
Description
Mode of action
Binds to ribosomal components and inhibits protein synthesis.
Mode of resistance
neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
