Neomycin

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==Experimental==
==Experimental==
 +
 +
===Target organism===
 +
bacteria, mammalian cells
===Stock solution===
===Stock solution===
 +
10mg/ml in water
 +
===Working solution===
===Working solution===
 +
10-50µg/ml
 +
 +
==Description==
 +
 +
===Mode of action===
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Binds to ribosomal components and inhibits protein synthesis.
 +
 +
===Mode of resistance===
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''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation.
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 +
==Related antibiotics==
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[[kanamycin]], [[G418]]
-
==Mode of action==
+
==External links==
 +
* list of cell culture reagents by Krackeler [http://www.krackeler.com/products/fid/2910]
 +
* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14]
 +
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin]

Revision as of 08:02, 4 August 2006

Contents

Experimental

Target organism

bacteria, mammalian cells

Stock solution

10mg/ml in water

Working solution

10-50µg/ml

Description

Mode of action

Binds to ribosomal components and inhibits protein synthesis.

Mode of resistance

neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.

Related antibiotics

kanamycin, G418

External links

  • list of cell culture reagents by Krackeler [1]
  • list of cell culture reagents by USB [2]
  • forum thread on difference between neomycin & G418 [3]
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