Neomycin: Difference between revisions
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(neo - beta 3 - reformating: too many small headings) |
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==Experimental== | ==Experimental== | ||
'''target organism''': bacteria, mammalian cells | *'''target organism''': bacteria, mammalian cells | ||
'''stock solution''': 10mg/ml in water | *'''stock solution''': 10mg/ml in water | ||
'''working solution''': 10-50µg/ml (stable at RT for 5days) | *'''working solution''': 10-50µg/ml (stable at RT for 5days) | ||
==Description== | ==Description== | ||
'''mode of action''': binds to ribosomal components and inhibits protein synthesis. | *'''mode of action''': binds to ribosomal components and inhibits protein synthesis. | ||
'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | *'''mode of resistance''': ''neo/kan'' resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates [[G418]], neomycin, and [[kanamycin]] by phosphorylation. | ||
==Links== | ==Links== | ||
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* list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | * list of cell culture reagents by USB [http://www.usbweb.com/reference2.asp?id_ref=14] | ||
* forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | * forum thread on difference between neomycin & G418 [http://www.protocol-online.org/forums/index.php?showtopic=10608&hl=neomycin] | ||
[[Category:Material]] [[Category:Antibiotic]] | |||
Latest revision as of 10:47, 26 July 2007
Experimental
- target organism: bacteria, mammalian cells
- stock solution: 10mg/ml in water
- working solution: 10-50µg/ml (stable at RT for 5days)
Description
- mode of action: binds to ribosomal components and inhibits protein synthesis.
- mode of resistance: neo/kan resistance gene is an aminoglycoside 3'-phosphotransferase, which inactivates G418, neomycin, and kanamycin by phosphorylation.
