Muratore:Materials/Media/LB: Difference between revisions

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#autoclave with caps loose
#autoclave with caps loose
#store long term at 4°C or for immediate use at room T
#store long term at 4°C or for immediate use at room T
====History:====
5/24/2011 by [[User:Kathryn Muratore|Kathryn Muratore]]


[[Category:Material]]
[[Category:Material]]
[[Category:Media]]
[[Category:Media]]

Latest revision as of 12:55, 16 August 2011

Bottles of 50 mL LB

  • 1 g Bacto-tryptone
  • 0.5 g Yeast extract
  • 1 g NaCl
  1. add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles
  2. autoclave with caps loose
  3. store long term at 4°C or for immediate use at room T

History:

5/24/2011 by Kathryn Muratore