Muratore:Materials/Media/LB

Bottles of 50 mL LB

• 1 g Bacto-tryptone
• 0.5 g Yeast extract
• 1 g NaCl

1. add water to 50 mL (use graduated cylinder) and transfer to 200 mL Wheaton bottles
2. autoclave with caps loose
3. store long term at 4°C or for immediate use at room T

History:

5/24/2011 by Kathryn Muratore