McClean: Phosphate Buffered Saline

Materials

• sodium chloride (NaCl)
• potassium chloride (KCl)
• sodium phosphate, dibasic (Na₂HPO₄)
• potassium phosphate, monobasic (KH₂PO₄)

Protocol

To make 1L of a 10x solution:

• Add together:
  • 80 g sodium chloride (NaCl)
  • 2 g potassium chloride (KCl)
  • 14.4 g sodium phophate, dibasic (Na₂HPO₄)
  • 2.4 g potassium phosphate, monobasic (KH₂PO₄)
• Adjust pH to 7.4 with HCl if necessary.
• Bring to 1 L with dH2O.
• Autoclave