MOPS: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Some OWW protocols which use MOPS) |
|||
| Line 14: | Line 14: | ||
* store at room temperature | * store at room temperature | ||
* protect from light; do not use if the solution appears yellow | * protect from light; do not use if the solution appears yellow | ||
==Some OWW protocols which use MOPS== | |||
* [[Jacobs:Protocol RNA Agarose Gel]] | |||
* [[Knight:NuPAGE electrophoresis]] | |||
* [[Sauer:bis-Tris SDS-PAGE, the very best]] | |||
Revision as of 08:33, 2 March 2009
Recipe for 10x MOPS buffer
- 41.2g 3-(N-morpholino) propanesulfonic acid (MOPS)
- 10.9g Sodium Acetate, 3- hydrate
- 3.7g EDTA, sodium salt
- add 800ml of nuclease free distilled water; mix to dissolve
- adjust to pH 7 with NaOH (prepared in nuclease free distilled water)
- fill to the final volume of 1000 ml
- filter sterilise
- store at room temperature
- protect from light; do not use if the solution appears yellow
