Lidstrom:Assaying enzyme libraries

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Janet B. Matsen (Talk | contribs)
(New page: Back to Protocols == Guiding Principles == * What range of detection do you want relative to your + control? ** If you are looking for high activity mutants, make s...)
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Back to Protocols

Guiding Principles

  • What range of detection do you want relative to your + control?
    • If you are looking for high activity mutants, make sure you aren't saturating the linear range of your detection capabilities.
    • If you are looking for lower activity mutants, make sure your lower limit of detection is suitable.
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