Koch Lab:Protocols/Kinesin/Tubulin resuspension and polymerization/BRB80
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BRB80 buffer consists of
- 1 mM MgCl2
- 1 mM EGTA
- 80 mM PIPES
- ph 6.9
Cytoskeleton calls this buffer PEM. When you add GTP to PEM, you get GPEM to use Cytoskeleton's lingo.
