Janet B. Matsen:Lab Tips & Tricks: Revision history

12 February 2014

• curprev 09:0109:01, 12 February 2014‎ Janet B. Matsen talk contribs‎ 26,646 bytes +429‎ →‎Buffers
• curprev 08:5808:58, 12 February 2014‎ Janet B. Matsen talk contribs‎ 26,217 bytes +16‎ →‎Software

14 October 2013

• curprev 18:1318:13, 14 October 2013‎ Janet B. Matsen talk contribs‎ 26,201 bytes +700‎ →‎Make your electrocompetent cells super viscous
• curprev 18:0918:09, 14 October 2013‎ Janet B. Matsen talk contribs‎ 25,501 bytes +9‎ →‎Nonunifrom plating is good for miniprepped plasmids you expect to yield decent transformation
• curprev 18:0818:08, 14 October 2013‎ Janet B. Matsen talk contribs‎ 25,492 bytes +21‎ →‎Nonunifrom plating is good for minipreps you expect to work decently well
• curprev 18:0718:07, 14 October 2013‎ Janet B. Matsen talk contribs‎ 25,471 bytes +6‎ →‎Plating transformations you expect will work

24 September 2013

• curprev 16:0216:02, 24 September 2013‎ Janet B. Matsen talk contribs‎ 25,465 bytes +89‎ →‎Using BLAST
• curprev 16:0016:00, 24 September 2013‎ Janet B. Matsen talk contribs‎ 25,376 bytes +369‎ →‎Using BLAST
• curprev 15:4915:49, 24 September 2013‎ Janet B. Matsen talk contribs‎ 25,007 bytes +97‎ →‎Using BLAST
• curprev 15:4415:44, 24 September 2013‎ Janet B. Matsen talk contribs‎ 24,910 bytes +4‎ →‎Using BLAST
• curprev 15:3615:36, 24 September 2013‎ Janet B. Matsen talk contribs‎ 24,906 bytes +54‎ →‎Using BLAST
• curprev 15:3315:33, 24 September 2013‎ Janet B. Matsen talk contribs‎ 24,852 bytes +1,136‎ →‎Using BLAST
• curprev 15:1315:13, 24 September 2013‎ Janet B. Matsen talk contribs‎ 23,716 bytes +1,084‎ →‎Using BLAST
• curprev 15:0215:02, 24 September 2013‎ Janet B. Matsen talk contribs‎ 22,632 bytes +399‎ →‎Software

23 August 2013

• curprev 15:1815:18, 23 August 2013‎ Janet B. Matsen talk contribs‎ 22,233 bytes +179‎ →‎Make your electrocompetent cells super viscous

31 March 2013

• curprev 08:1808:18, 31 March 2013‎ Janet B. Matsen talk contribs‎ 22,054 bytes +579‎ →‎Transformations
• curprev 08:0708:07, 31 March 2013‎ Janet B. Matsen talk contribs‎ 21,475 bytes −1‎ →‎Nonunifrom plating is good for minipreps you expect to work decently well
• curprev 08:0708:07, 31 March 2013‎ Janet B. Matsen talk contribs‎ 21,476 bytes +644‎ →‎Transformations

28 March 2013

• curprev 12:5112:51, 28 March 2013‎ Janet B. Matsen talk contribs‎ 20,832 bytes +372‎ →‎Gels
• curprev 11:2311:23, 28 March 2013‎ Janet B. Matsen talk contribs‎ 20,460 bytes +758‎ →‎Tech Support

27 March 2013

• curprev 12:0612:06, 27 March 2013‎ Janet B. Matsen talk contribs‎ 19,702 bytes +199‎ →‎Keep an extra clean set up pipette tips
• curprev 12:0512:05, 27 March 2013‎ Janet B. Matsen talk contribs‎ 19,503 bytes +375‎ →‎Keep an extra clean set up pipette tips

22 March 2013

• curprev 10:3310:33, 22 March 2013‎ Janet B. Matsen talk contribs‎ 19,128 bytes −3‎ →‎Keep an extra clean set up pipette tips
• curprev 10:2710:27, 22 March 2013‎ Janet B. Matsen talk contribs‎ 19,131 bytes +354‎ →‎Keep an extra clean set up pipette tips

12 February 2013

• curprev 08:4608:46, 12 February 2013‎ Janet B. Matsen talk contribs‎ 18,777 bytes −16‎ →‎Tech Support Angels & Demons
• curprev 08:4608:46, 12 February 2013‎ Janet B. Matsen talk contribs‎ 18,793 bytes 0‎ →‎+= Using APE (free plasmid editing software) ==
• curprev 08:4608:46, 12 February 2013‎ Janet B. Matsen talk contribs‎ 18,793 bytes +1,012‎ →‎Using APE (free plasmid editing software)

31 January 2013

• curprev 14:0314:03, 31 January 2013‎ Janet B. Matsen talk contribs‎ m 17,781 bytes +13‎ →‎Transformations
• curprev 14:0114:01, 31 January 2013‎ Janet B. Matsen talk contribs‎ 17,768 bytes +738‎ →‎Equipment
• curprev 13:5713:57, 31 January 2013‎ Janet B. Matsen talk contribs‎ m 17,030 bytes +2‎ →‎Minipreps

24 January 2013

• curprev 14:2614:26, 24 January 2013‎ Janet B. Matsen talk contribs‎ 17,028 bytes +100‎ →‎Numbering for Experiments
• curprev 14:2514:25, 24 January 2013‎ Janet B. Matsen talk contribs‎ 16,928 bytes +196‎ →‎Numbering for Experiments
• curprev 14:2414:24, 24 January 2013‎ Janet B. Matsen talk contribs‎ m 16,732 bytes +2‎ →‎Keep an extra clean set up pipette tips
• curprev 14:2314:23, 24 January 2013‎ Janet B. Matsen talk contribs‎ 16,730 bytes +402‎ →‎Sterile Technique
• curprev 13:3613:36, 24 January 2013‎ Janet B. Matsen talk contribs‎ 16,328 bytes +892‎ →‎Sterile Technique
• curprev 13:3013:30, 24 January 2013‎ Janet B. Matsen talk contribs‎ 15,436 bytes −145‎ →‎Tech Support Angels & Demons
• curprev 13:2813:28, 24 January 2013‎ Janet B. Matsen talk contribs‎ 15,581 bytes +253‎ →‎Equipment
• curprev 11:0311:03, 24 January 2013‎ Janet B. Matsen talk contribs‎ 15,328 bytes +466‎ →‎Media

9 January 2013

• curprev 14:4714:47, 9 January 2013‎ Janet B. Matsen talk contribs‎ 14,862 bytes +1,214‎ No edit summary

18 December 2012

• curprev 21:1521:15, 18 December 2012‎ Janet B. Matsen talk contribs‎ 13,648 bytes +158‎ →‎Transformations
• curprev 21:1321:13, 18 December 2012‎ Janet B. Matsen talk contribs‎ m 13,490 bytes +311‎ →‎Equipment
• curprev 19:3919:39, 18 December 2012‎ Janet B. Matsen talk contribs‎ m 13,179 bytes +16‎ No edit summary
• curprev 19:0119:01, 18 December 2012‎ Janet B. Matsen talk contribs‎ 13,163 bytes +44‎ No edit summary
• curprev 18:5918:59, 18 December 2012‎ Janet B. Matsen talk contribs‎ 13,119 bytes +371‎ No edit summary

29 October 2012

• curprev 10:4910:49, 29 October 2012‎ Janet B. Matsen talk contribs‎ 12,748 bytes +1,329‎ →‎Using APE (free plasmid editing software)

10 October 2012

• curprev 09:5209:52, 10 October 2012‎ Janet B. Matsen talk contribs‎ 11,419 bytes −77‎ No edit summary
• curprev 09:0409:04, 10 October 2012‎ Janet B. Matsen talk contribs‎ 11,496 bytes +159‎ →‎DNA Magic
• curprev 09:0109:01, 10 October 2012‎ Janet B. Matsen talk contribs‎ 11,337 bytes +979‎ →‎PCR

8 October 2012

• curprev 14:5814:58, 8 October 2012‎ Janet B. Matsen talk contribs‎ m 10,358 bytes +47‎ →‎Eluting DNA from miniprep & gel extraction columns
• curprev 14:4114:41, 8 October 2012‎ Janet B. Matsen talk contribs‎ 10,311 bytes −1‎ →‎Eluting DNA from miniprep & gel extraction columns
• curprev 14:4014:40, 8 October 2012‎ Janet B. Matsen talk contribs‎ m 10,312 bytes +138‎ →‎Eluting DNA from miniprep & gel extraction columns
• curprev 14:3614:36, 8 October 2012‎ Janet B. Matsen talk contribs‎ 10,174 bytes +25‎ →‎PCR product purification
• curprev 14:3514:35, 8 October 2012‎ Janet B. Matsen talk contribs‎ 10,149 bytes +924‎ No edit summary

3 October 2012

• curprev 15:3215:32, 3 October 2012‎ Janet B. Matsen talk contribs‎ 9,225 bytes +356‎ →‎Misc
• curprev 15:0315:03, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,869 bytes +314‎ →‎Media
• curprev 11:3011:30, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,555 bytes +30‎ →‎The strain you use really matters!
• curprev 10:4710:47, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,525 bytes +16‎ →‎DNA Magic
• curprev 10:4610:46, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,509 bytes +12‎ →‎The strain you use really matters!
• curprev 10:4410:44, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,497 bytes +62‎ →‎Gels
• curprev 10:4210:42, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,435 bytes +22‎ →‎Grow overnight cultures with sufficient head space
• curprev 10:4210:42, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,413 bytes +33‎ →‎Grow overnight cultures in liquid:air ratios of at least 1:4
• curprev 10:4110:41, 3 October 2012‎ Janet B. Matsen talk contribs‎ 8,380 bytes +772‎ →‎Minipreps

2 October 2012

• curprev 09:5909:59, 2 October 2012‎ Janet B. Matsen talk contribs‎ 7,608 bytes +13‎ →‎Gels
• curprev 09:5809:58, 2 October 2012‎ Janet B. Matsen talk contribs‎ 7,595 bytes +6‎ →‎Gels
• curprev 09:5709:57, 2 October 2012‎ Janet B. Matsen talk contribs‎ 7,589 bytes −13‎ →‎Gels
• curprev 09:5309:53, 2 October 2012‎ Janet B. Matsen talk contribs‎ 7,602 bytes +382‎ →‎Gels

21 August 2012

• curprev 09:3209:32, 21 August 2012‎ Janet B. Matsen talk contribs‎ 7,220 bytes +6‎ →‎Misc
• curprev 09:3109:31, 21 August 2012‎ Janet B. Matsen talk contribs‎ 7,214 bytes +149‎ →‎Transformations
• curprev 09:2809:28, 21 August 2012‎ Janet B. Matsen talk contribs‎ 7,065 bytes +64‎ →‎Transformations
• curprev 09:2609:26, 21 August 2012‎ Janet B. Matsen talk contribs‎ 7,001 bytes +490‎ →‎Transformations
• curprev 09:2409:24, 21 August 2012‎ Janet B. Matsen talk contribs‎ 6,511 bytes 0‎ →‎Equipment
• curprev 09:2409:24, 21 August 2012‎ Janet B. Matsen talk contribs‎ 6,511 bytes 0‎ →‎DNA Magic

2 August 2012

• curprev 17:5517:55, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,511 bytes +7‎ →‎Equipment
• curprev 17:5417:54, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,504 bytes −97‎ →‎Equipment
• curprev 17:5317:53, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,601 bytes 0‎ →‎Equipment
• curprev 17:5217:52, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,601 bytes +45‎ →‎Equipment
• curprev 17:5117:51, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,556 bytes +15‎ →‎Equipment
• curprev 17:5117:51, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,541 bytes −91‎ →‎Equipment
• curprev 17:5017:50, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,632 bytes +155‎ →‎Equipment
• curprev 17:4617:46, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,477 bytes +2‎ →‎Equipment
• curprev 17:4517:45, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,475 bytes +151‎ No edit summary
• curprev 09:4009:40, 2 August 2012‎ Janet B. Matsen talk contribs‎ 6,324 bytes +474‎ →‎Keeping primers, plasmids, strain stocks, & sequencing organized
• curprev 09:3809:38, 2 August 2012‎ Janet B. Matsen talk contribs‎ 5,850 bytes +496‎ →‎Keeping primers, plasmids, strain stocks, & sequencing organized
• curprev 09:3509:35, 2 August 2012‎ Janet B. Matsen talk contribs‎ 5,354 bytes +125‎ No edit summary
• curprev 09:3409:34, 2 August 2012‎ Janet B. Matsen talk contribs‎ 5,229 bytes +317‎ No edit summary
• curprev 09:3209:32, 2 August 2012‎ Janet B. Matsen talk contribs‎ 4,912 bytes +276‎ No edit summary
• curprev 09:3109:31, 2 August 2012‎ Janet B. Matsen talk contribs‎ 4,636 bytes +180‎ No edit summary

3 July 2012

• curprev 17:1217:12, 3 July 2012‎ Janet B. Matsen talk contribs‎ 4,456 bytes +592‎ →‎DNA Magic

2 March 2012

• curprev 14:2414:24, 2 March 2012‎ Janet B. Matsen talk contribs‎ 3,864 bytes +81‎ →‎Media
• curprev 14:2314:23, 2 March 2012‎ Janet B. Matsen talk contribs‎ 3,783 bytes +23‎ →‎Media
• curprev 14:2214:22, 2 March 2012‎ Janet B. Matsen talk contribs‎ 3,760 bytes +566‎ No edit summary
• curprev 14:1814:18, 2 March 2012‎ Janet B. Matsen talk contribs‎ 3,194 bytes +1,430‎ No edit summary

19 February 2012

• curprev 15:4115:41, 19 February 2012‎ Janet B. Matsen talk contribs‎ 1,764 bytes +298‎ →‎DNA Magic

10 February 2012

• curprev 18:1918:19, 10 February 2012‎ Janet B. Matsen talk contribs‎ 1,466 bytes +1‎ No edit summary
• curprev 18:1818:18, 10 February 2012‎ Janet B. Matsen talk contribs‎ 1,465 bytes +21‎ No edit summary
• curprev 18:1518:15, 10 February 2012‎ Janet B. Matsen talk contribs‎ 1,444 bytes +51‎ No edit summary
• curprev 18:1018:10, 10 February 2012‎ Janet B. Matsen talk contribs‎ 1,393 bytes −290‎ No edit summary
• curprev 18:1018:10, 10 February 2012‎ Janet B. Matsen talk contribs‎ 1,683 bytes +276‎ No edit summary

7 February 2012

• curprev 13:5513:55, 7 February 2012‎ Janet B. Matsen talk contribs‎ 1,407 bytes +101‎ No edit summary
• curprev 13:5513:55, 7 February 2012‎ Janet B. Matsen talk contribs‎ 1,306 bytes +239‎ No edit summary
• curprev 13:5013:50, 7 February 2012‎ Janet B. Matsen talk contribs‎ 1,067 bytes +1,067‎ New page: Back to Janet This collection is mostly from advice given to me by The Amazing Justin Siegel. == DNA Magic == *purifying DNA on a gel column has ~ 90% loss. pu...