IGEM:metu/2009/Notebook/wound dressing/2009/09/03
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 WOUND DRESSING
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03.09.20091. We couldn't make isolation. 2. Electrophoresis order; 1)leader / c1/ 1a / 1b / c2 / 2a / 2b / c4 / 4a / 4b /empty / 8a / 8b /c8 3. Digestions of 1,2 and 4 were made. The following amounts are added to microtubes; 1a 1b
1 , Buffer 4 µl 4 µl
Spe1 4 µl EcoR1 4 µl
DNA 28 µl 27 µl
water 4 µl =40 µl 7 µl =42 µl
2a 2b
2 , Buffer 4 µl 4 µl
Pst1 4 µl Xba1 4 µl
DNA 28 µl 26.5 µl
water 4 µl =40 µl 7.5 µl =42 µl
4a 4b
4 , Buffer 4 µl 4 µl
Spe1 4 µl EcoR1 4 µl
DNA 27 µl 25 µl
water 7 µl =42 µl 9 µl =42 µl
4. We coldn't digest.
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