IGEM:metu/2009/Notebook/wound dressing/2009/09/03

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Image: Wound dressing logo.jpg

03.09.2009

1. We couldn't make isolation.

2. Electrophoresis order;

  1)leader / c1/  1a / 1b / c2 / 2a / 2b / c4 / 4a / 4b /empty / 8a / 8b /c8 

3. Digestions of 1,2 and 4 were made. The following amounts are added to microtubes;

                                       1a                                   1b
   1 ,         Buffer                  4 µl                                 4  µl
               Spe1                    4 µl                   EcoR1         4  µl
               DNA                     28 µl                                27 µl
               water                   4 µl     =40 µl                      7 µl       =42 µl
                                       2a                                   2b
   2 ,         Buffer                  4 µl                                 4  µl
               Pst1                    4 µl                   Xba1          4  µl
               DNA                     28 µl                                26.5 µl
               water                   4 µl     =40 µl                      7.5 µl     =42 µl
                                       4a                                   4b
   4 ,         Buffer                  4 µl                                 4 µl
               Spe1                    4 µl                   EcoR1         4 µl
               DNA                     27 µl                                25 µl
               water                   7 µl     =42 µl                      9 µl       =42 µl

4. We coldn't digest.



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