IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Week Twelve</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Floor One== | ||
Made 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates. | |||
Isolated more single colonies that resembled Streptomyces from soil sample plates. | |||
==Floor Two== | |||
We inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C. | |||
Latest revision as of 23:11, 26 September 2017
 Week Twelve
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Floor OneMade 6 x 500 ml SFM agar media, autoclaved for 1 hr and poured into plates. Isolated more single colonies that resembled Streptomyces from soil sample plates.
Floor TwoWe inoculated cultures of BL21 containing pETAntA and Antgp+RFP into 10mls LB with antibiotics Kanamycin and Chlorophenicol. Four cultures were grown overnight at 37°C.
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