IGEM:University of East Anglia (UEA), Norwich, UK/2009/Notebook/NRP-UEA-Norwich iGEM/2013/08/14: Difference between revisions
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==Floor One== | |||
De-replication of single Streptomyces colony plates where it appeared we had duplicates. | |||
==Floor Two== | ==Floor Two== | ||
As the BL21 plates were unsuccessful, we decided to make some new competent cells, following the protocol. These cells were then transformed with Pet28AntA and spread onto plates for incubation overnight at 37°C. <br> | As the BL21 plates were unsuccessful, we decided to make some new competent cells, following the protocol. These cells were then transformed with Pet28AntA and spread onto plates for incubation overnight at 37°C. <br> |
Revision as of 07:43, 22 August 2013
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Floor OneDe-replication of single Streptomyces colony plates where it appeared we had duplicates. Floor TwoAs the BL21 plates were unsuccessful, we decided to make some new competent cells, following the protocol. These cells were then transformed with Pet28AntA and spread onto plates for incubation overnight at 37°C.
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