IGEM:UNAM Genomics Mexico/2009/Notebook/Wifi coli/2010/05/13: Difference between revisions
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*Make ligation for promoter and double terminator. | *Make ligation for promoter and double terminator. | ||
*Get Luciferase, get mutant and clone it. | *Get Luciferase, get mutant and clone it. | ||
*Primers used for blue promoter: | *Primers used for blue promoter: [http://partsregistry.org/Part:BBa_K238013:Experience BBa_K238013] | ||
==Modeling== | ==Modeling== |
Revision as of 17:17, 15 May 2010
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13th may 2010WetLabExtraction of genomic DNA for blue promoter is done, but we don´t have amplified. Goals for next week
ModelingWe´ve got the primary function for activity of Luciferase and concentration of cytosolic Luciferin. Goals for next week
Required data
propose, an association between promoter and quantity of reporter. Search protocols with measure about light and concentration. TRpR → aλ[P1] Promoter of Blue receptor → bλ[P2] Where λ is light. Ps, concentrations.
General
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