IGEM:Peking/2007: Difference between revisions
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<div style="font: normal 1.6em georgia, serif">Towards Self-differentiated Bacterial Assembly Line</div> | |||
Our projects concern with the ability for bacterial cells to '''differentiate''' out of homogeneous conditions into populations with the '''division of labor'''. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for '''temporal''' and '''spatial''' differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome. | |||
<br> | |||
About the name of projects, the two devices are named after their respective functions: hop count and push-on-push-off switch, but they are common for breaking symmetry, which is interesting and ubiquitous in science. Although this "differentiation" process resemble the development of multicellular organism, we tend to use a more bioengineering style analogy: assembly line. Or maybe after some years from now, this will not be just an analogy. <br> | |||
'''Also visit:''' [http://parts.mit.edu/igem07/index.php/Peking '''IGEM2007 Peking'''] | |||
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![http://parts.mit.edu/igem07/index.php/Peking IGEM 2007 Peking] | |||
![http://parts.mit.edu/igem07/index.php/Peking_The_Projects Peking The Projects] | |||
![http://parts.mit.edu/igem07/index.php/Peking_The_Community Peking The Team] | |||
![http://parts.mit.edu/igem07/index.php/Peking_The_Fun Peking's Misc & Fun] | |||
![http://parts.mit.edu/igem07/index.php/Peking_Thanks Acknowledgement] | |||
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<img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> | <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> | ||
</html>Project | </html>Project== | ||
'''Hop Count with Conjugation'''<br> | '''Hop Count with Conjugation'''<br> | ||
[ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/proposal.pdf Project Proposal]<br> | [ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/proposal.pdf Project Proposal]<br> | ||
[ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/presentation.pdf Project Presentation]<br> | [ftp://igem:igem2007@162.105.160.5/OurFiles/CountGroup/Documents/presentation.pdf Project Presentation]<br> | ||
[[ | [[iGEM:Peking/2007/Count-Road-Map| Road Map ]]<br> | ||
[[ | [[iGEM:Peking/2007/Count-References | Related Articles ]]<br> | ||
'''Push | '''Push-on-push-off Switch'''<br> | ||
Project proposal<br> | [ftp://igem:igem2007@162.105.160.5/OurFiles/SwitchGroup/proposal_switch_revision2.doc Project proposal]<br> | ||
Project presentation<br> | Project presentation<br> | ||
[[ | [[iGEM:Peking/2007/Switch-Road-Map | Road Map ]]<br> | ||
[[ | [[iGEM:Peking/2007/Switch-References | Related Articles ]]<br> | ||
'''Meetings''' | '''Meetings''' | ||
* [[ | * [[iGEM:Peking/2007/iGEM-Promotion-Meeting|iGEM promotion meeting]] | ||
* Journal Club [[ | * [[iGEM:Peking/2007/iGEM-Beijing-Preparation-Meeting|iGEM PKU prep meeting]] | ||
* Journal Club [[iGEM:Peking/2007/JC01|01]] | [[iGEM:Peking/2007/JC02|02]] | [[iGEM:Peking/2007/JC03|03]] | [[iGEM:Peking/2007/JC04|04]] | [[iGEM:Peking/2007/JC05|05]] | [[iGEM:Peking/2007/JC06|06]] | [[iGEM:Peking/2007/JC07|07]] | [[iGEM:Peking/2007/JC08|08]] | [[iGEM:Peking/2007/JC09|09]] | [[iGEM:Peking/2007/JC10|10]] | [[iGEM:Peking/2007/JC11|11]] | [[iGEM:Peking/2007/JC12|12]] | [[iGEM:Peking/2007/JC13|13]] | [[iGEM:Peking/2007/JC17|17]] | |||
'''Brainstorming'''<br> | '''Brainstorming'''<br> | ||
Some of our ideas are listed [[ | Some of our ideas are listed [[iGEM:Peking/2007/Ideas | here]] | ||
'''Rap up'''<br> | |||
Collections of the experiences. | |||
* A [[IGEM:Peking/2007/Experience-colletcion | response]] to Goodman. In English. | |||
* Collections of [[IGEM:Peking/2007/Things_we_want_to_say |chinese sentiments]]. | |||
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<a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.org/wiki/IGEM:Peking/2007"><img src="http://www3.clustrmaps.com/stats/maps-no_clusters/www.openwetware.org-wiki-IGEM-Peking-2007-thumb.jpg" /> | |||
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<img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> | <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> | ||
</html>Lab< | </html>Lab== | ||
< | |||
'''Products and Parts''' | |||
'''Procedures'''<br> | |||
[[iGEM:Peking/2007/Count-Conjugation-Procedure |Hop-count with Conjugation]]<br> | |||
[[iGEM:Peking/2007/Switch-Procedure |Push-on-push-off Switch]]<br> | |||
''' | '''Protocols'''<br> | ||
[[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Count:Agarose Gel Electrophoresis]]<br> | |||
[[iGEM:Peking/2007/Count:Gel-Extraction | Count:DNA Gel Extraction]]<br> | |||
[[iGEM:Peking/2007/Count:PCR product purifcation| Count:PCR product purifcation]]<br> | |||
[[iGEM:Peking/2007/Count:Preparation of Competent Cells | Count:Preparation of Competent Cells]]<br> | |||
[[iGEM:Peking/2007/Digestion | Enzymatic DNA Digestion]]<br> | |||
[[iGEM:Peking/2007/Count:Ligation | Count:Ligation]]<br> | |||
[[iGEM:Peking/2007/Count:Transformation | Count:Transformation]]<br> | |||
[[iGEM:Peking/2007/Count:Sequencing | Count:Sequencing]]<br> | |||
[[iGEM:Peking/2007/Count:Miniprep | Count: Miniprep]]<br> | |||
[[iGEM:Peking/2007/Count:Conjugation| Count: Conjugation test]]<br> | |||
[[iGEM:Peking/2007/Count:Knockout| Count: Gene Knock Out]]<br> | |||
[[iGEM:Peking/2007/Switch:Miller | Switch: Miller Method]]<br> | |||
[[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br> | |||
[[iGEM:Peking/2007/Switch: PCR | Switch: PCR]]<br> | |||
[[iGEM:Peking/2007/Switch: DNA electrophoresis | Switch: DNA electrophoresis]]<br> | |||
[[iGEM:Peking/2007/Switch: DNA ligation | Switch: DNA ligation]]<br> | |||
[[iGEM:Peking/2007/Switch: DNA digestion | Switch: DNA digestion]]<br> | |||
[[iGEM:Peking/2007/Switch: transformation | Switch: transformation]]<br> | |||
[[iGEM:Peking/2007/Switch: DNA rescue by precipitation | Switch: DNA rescue by precipitation]]<br> | |||
[[iGEM:Peking/2007/Switch: Plasmid mini prep| Switch: Plasmid mini prep]]<br> | |||
[[Image:Peking_2007_Notebook_icon.jpg|20px]]'''Notebooks'''<br> | |||
[[iGEM:Peking/2007/Count-Conjugation-Notebook |Hop-count with Conjugation]]<br> | |||
[[iGEM:Peking/2007/Switch_Notebook|Push-on-push-off Switch]]<br> | |||
[[iGEM:Peking/2007/Turtle_Diary|Turtle Diary]]<br> | |||
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<img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> | <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> | ||
</html>People | </html>People== | ||
The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. | The iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. | ||
=== Students === | === Students === | ||
*[[iGEM:Peking/2007/Anting Xu | Anting Xu]] | |||
*[[iGEM:Peking/2007/Chang Liu | Chang Liu]] | |||
*[[iGEM:Peking/2007/Chongyi Chen | Chongyi Chen]] | |||
*[[iGEM:Peking/2007/Chunbo Lou | Chunbo Lou]] | |||
*[[User:Chen Daizhuo | Chen Daizhuo, Henry]] | |||
*[[iGEM:Peking/2007/Dan Lu | Dan Lu]] | |||
*[[iGEM:Peking/2007/Haisu Ma | Haisu Ma]] | |||
*[[iGEM:Peking/2007/Lingli Jiang | Lingli Jiang]] | |||
*[[iGEM:Peking/2007/Ming Ni | Ming Ni]] | |||
*[[iGEM:Peking/2007/Mingcong Wang | Mingcong Wang]] | |||
*[[Peking_Mingzhi Qu | Mingzhi Qu]] | |||
*[[iGEM:Peking/2007/Qinsi Zheng | Qinsi Zheng]] | |||
*[[iGEM:Peking/2007/Tao Ma | Tao Ma]] | |||
*[[iGEM:Peking/2007/Tao Yu | Tao Yu ]] | |||
*[[iGEM:Peking/2007/Ting Liu | Ting Liu]] | |||
*[[iGEM:Peking/2007/Xili Liu | Xili Liu]] | |||
*[[User:YangYifan | Yifan Yang]] | |||
*[[iGEM:Peking/2007/Zhe Ren | Zhe Ren]] | |||
=== Advisors === | === Advisors === | ||
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=== Photo === | === Photo === | ||
[[ | [[iGEM:Peking/2007/Photo-Team | The Team]] | ||
[[iGEM:Peking/2007/Photo-Campus | Our Campus]] | |||
[[ | [[iGEM:Peking/2007/Photo-Lab | The Labs]] | ||
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'''Email us''' at ''igem-pku AT googlegroups DOT com'' | '''Email us''' at ''igem-pku AT googlegroups DOT com'' | ||
'''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem- | '''External links''' [http://parts.mit.edu/wiki/index.php/Main_Page iGEM] | [http://www.igem2006.com iGEM 2006] | [http://parts.mit.edu/ Registry of Standard Biological Parts] | [http://groups.google.com/group/igem-pku/topics?lnk=oa iGEM PKU Googlegroup] | [[iGEM|iGEM OWW Page]] | ||
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Latest revision as of 14:11, 21 November 2007
Towards Self-differentiated Bacterial Assembly Line
Our projects concern with the ability for bacterial cells to differentiate out of homogeneous conditions into populations with the division of labor. We aim at devices conferring host cells with the ability to form cooperating groups spontaneously and to take consecutive steps sequentially even when the genetic background and environmental inputs are identical. To break the mirror in such homogeneous condition, we need two devices respectively responsible for temporal and spatial differentiation. The implementation and application of such devices will lead to a picture of future bioengineering where complex programs consisted of sequential steps (structure oriented programs) and cooperating agencies (forked instances of a single class, object and event oriented) can be embedded in a single genome.
|
IGEM 2007 Peking | Peking The Projects | Peking The Team | Peking's Misc & Fun | Acknowledgement |
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<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> </html>ProjectHop Count with Conjugation Push-on-push-off Switch Meetings
Brainstorming Rap up
<html> <a href="http://www3.clustrmaps.com/counter/maps.php?url=http://www.openwetware.org/wiki/IGEM:Peking/2007"><img src="http://www3.clustrmaps.com/stats/maps-no_clusters/www.openwetware.org-wiki-IGEM-Peking-2007-thumb.jpg" /> </a> </html> |
<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>LabProducts and Parts Procedures Protocols
|
<html> <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>PeopleThe iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. Students
Advisors
Photo |
Email us at igem-pku AT googlegroups DOT com
External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page