IGEM:Melbourne/2008/Notebook/BioClock/2008/08/05: Difference between revisions
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Alex Caputo (talk | contribs) (Autocreate 2008/08/05 Entry for IGEM:Melbourne/2008/Notebook/BioClock) |
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==Done today:== | ==Done today:== | ||
* | * Red light retest. | ||
4 transformations: | |||
*Cph8 + pPL-PCB | |||
*Cph8 | |||
*PCB | |||
*-ve control | |||
Protocol: | |||
*2μL DNA | |||
*RU1012 | |||
*30 min ice | |||
*1 min 42°C | |||
*1 hour at 37°C + LB | |||
Each transformation plated onto 2 plates, one of which is wrapped in foil. | |||
Placed in 37°C room at genetics which light on | |||
Plates: | |||
Amp | |||
Kan | |||
Chlor | |||
Arabinose (2mM) | |||
IPTG (1mM) | |||
X-gal | |||
Alex | |||
Revision as of 01:51, 5 August 2008
 Melbourne iGEM 2008 -- BioClock
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Done today:
4 transformations:
Each transformation plated onto 2 plates, one of which is wrapped in foil. Placed in 37°C room at genetics which light on Plates: Amp Kan Chlor Arabinose (2mM) IPTG (1mM) X-gal Alex
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