IGEM:IMPERIAL/2008/New/Chassis 1: Difference between revisions
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{{Imperial/ | {{Imperial/Box1|Motility|To achieve accurate distribution of our biofabricators, we must exert fine control over their motility. Bacteria's primary method of getting about is via flagellar locomotion. A protein ring on the cell membrane is attached to the flagellum and rotates during locomotion, acting like a propeller to push the cell through its environment. | ||
The precise mechanism of how this works in ''B. subtilis'' has recently been elucidated. The flagella can be detached from the rotor by expression of a clutch molecule that interacts with the flagella and distorts it, so it is disengaged from the rotor protein. Control over the expression of this clutch should allow us to control the bacteria very quickly. When we want the bacteria to stop, we trigger expression of the clutch, which halts movement. | The precise mechanism of how this works in ''B. subtilis'' has recently been elucidated. The flagella can be detached from the rotor by expression of a clutch molecule that interacts with the flagella and distorts it, so it is disengaged from the rotor protein. Control over the expression of this clutch should allow us to control the bacteria very quickly. When we want the bacteria to stop, we trigger expression of the clutch, which halts movement. | ||
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{{Imperial/ | {{Imperial/Box1|Biomaterial Synthesis|After our bacteria are positioned correctly, they need to express a biomaterial. ''B. subtilis'' is Gram-positive, meaning it has only a single membrane as opposed to a double membrane in Gram-negative bacteria like ''E. coli''. This means that the expression of a biomaterial is a lot more tractable; biomaterial can be produced and secreted more efficiently. With a double membrane, material may accumulate inside the cell and the efficiency of biomaterial production can be significantly lower. | ||
Another important aspect of our biomaterial specifications is what we want to secrete. We did a lot of research on this and decided to express elastin peptides and EAK16-II. Both are small peptides and their molecular structures favour their self-assembly outside the ''B. subtilis'' cells to form 3D bio-scaffolds. | Another important aspect of our biomaterial specifications is what we want to secrete. We did a lot of research on this and decided to express elastin peptides and EAK16-II. Both are small peptides and their molecular structures favour their self-assembly outside the ''B. subtilis'' cells to form 3D bio-scaffolds. |
Revision as of 09:32, 26 September 2008
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