IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

Super Parts	Molecular Prey-Predator Oscillator
Actual Part
Sub Parts	Test Sensing Predator Construct	Test Killing Predator Construct

• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation

Ligation strategy

Ligating the Polycystronic Predator Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4
<bbpart>J37023</bbpart> (<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
<bbpart>J37025</bbpart>= (<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37036</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)

Ligating the Monocystronic Predator Construct:

For	Stage 1	Stage 2	Stage 3	Stage 4	Stage 5
<bbpart>J37023</bbpart> (<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>= (<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>= (<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>= (<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>= (<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>= (<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>= (<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

• More information on ligations can be found on the Ligation Page.

Quality control

Part	Logo	Built	Sequenced	Primers Used for Sequencing
<bbpart>J37018</bbpart>		No	N/A	N/A
<bbpart>J37019</bbpart>		Yes	Yes	Primers used
<bbpart>J37023</bbpart>		Yes	Yes Note: This part was sequenced as a part of J37025	Primers used
<bbpart>J37024</bbpart>		No	N/A	N/A
<bbpart>J37025</bbpart>		Yes	Yes	Primers used
<bbpart>J37031</bbpart>		Yes	No	N/A
<bbpart>J37033</bbpart>		Yes	No	N/A
<bbpart>J37035</bbpart>		No	N/A	N/A
<bbpart>J37036</bbpart>		No	N/A	N/A

WetLab work entries

• the final assembly was not successful
• The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
• Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).