IGEM:IMPERIAL/2006/Protocols: Difference between revisions
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*[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]] | *[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]] | *[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 uL Digestion]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 {{uL}} Digestion]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]] | *[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]] | *[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]] | ||
Revision as of 05:38, 28 October 2006
Protocols
- Bacterial Culture
- LB Amp Plate
- Transformations
- Competent Cells
- Bradford Assay
- 20 μL Digestion
- 20 μL Ligation
- 30 μL Ligation
- Miniprep of Culture
- 50x TAE Solution
- Agarose Gel
- Measuring DNA Concentration
- Fusion PCR protocol
- Western Blot Protocol
- 1 kb Plus DNA Ladder
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria)
