IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/07: Difference between revisions

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[[Image: Chewbacca.jpg||500px]]
[[Image: Chewbacca.jpg||500px]]
*  Can he handle the flavor?  Chew on that question.
===Sequencing===
===Sequencing===
V0120 Plasmids containing the pENTCUP2 plant promoter, NOS terminator and NOS terminator + STOP were sent to GENEWIZ for sequencing.  Sequencing results are expected tomorrow.
V0120 Plasmids containing the pENTCUP2 plant promoter, NOS terminator and NOS terminator + STOP were sent to GENEWIZ for sequencing.  Sequencing results are expected tomorrow.

Revision as of 08:11, 7 July 2010

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Team Flavor

  • Can he handle the flavor? Chew on that question.

Sequencing

V0120 Plasmids containing the pENTCUP2 plant promoter, NOS terminator and NOS terminator + STOP were sent to GENEWIZ for sequencing. Sequencing results are expected tomorrow.

Sequencing Order


Cultures

5 mL cultures were started from the YFP-2x construct from yesterday, as well as the B15 (StrepII) tag.

  • 2 x B21 E/X + Brazz E/S - C-Terminus
  • 2 x B21 S/P + Brazz X/P - N-Terminus
  • 2 x B21 E/X + Mira E/S - C-Terminus
  • 2 x B21 S/P + Mira X/P - N-Terminus
  • 2 x B15 Plate #1
  • 2 x B15 Plate #2

Cultures were placed in a 37°C incubator and left to shake overnight.



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