IGEM:Harvard/2007/Protocols/Fluorescent Labeling Protocol

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Revision as of 07:07, 13 July 2007 by KMagic246 (talk | contribs) (New page: #Resuspend up to 10^7 nucleated cells in 100 ul of buffer. #Add 10 ul of MACS Fluorochrome-conjugated Antibodies. #Mix well and refrigerate for 10 minutes in the dark. #Wash cells by addin...)
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  1. Resuspend up to 10^7 nucleated cells in 100 ul of buffer.
  2. Add 10 ul of MACS Fluorochrome-conjugated Antibodies.
  3. Mix well and refrigerate for 10 minutes in the dark.
  4. Wash cells by adding 1-2 ml of buffer per 10^7 cells and centrifuge at 300 x g for 10 minutes. Aspirate supernatant completely.
  5. Resuspend cell pellet in a suitable amount of buffer for analysis by flow cytometry or fluorescence microscopy.
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