IGEM:Groningen/Notebook/iGEM 2011/2011/06/01: Difference between revisions

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==Entry title==
==01-06-11==
* Insert content here...
 
<br>Do a PCR for PhybB on E.coli DH5alpha suspension:
<br>Composition:
<br>Taq 10× buffer: 5μl
<br>dNTPs: 1μl
<br>MgCl2: 3μl
<br>taq 5u/μl: 0.25μl
<br>Forward primer 10mM: 1μl
<br>Reverseprimer 10mM: 1μl
<br>Template: 1μl
<br>MQ: 38.75μl
<br>
<br>Analyse them on a 1% agarosegel
<br>PCR conditions:
<br>denaturation: 10min
<br>Cycle 25×
<br>denaturtaion: 1min
<br>annealing: 1min (gradient for 60, 65 and 70 degrees)
<br>extension: 1min
<br>Final extension: 10min
<br>Store infinite
<br>
<br> Making cloning strategy with biobrick parts
<br>




Revision as of 01:29, 21 September 2011

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01-06-11


Do a PCR for PhybB on E.coli DH5alpha suspension:
Composition:
Taq 10× buffer: 5μl
dNTPs: 1μl
MgCl2: 3μl
taq 5u/μl: 0.25μl
Forward primer 10mM: 1μl
Reverseprimer 10mM: 1μl
Template: 1μl
MQ: 38.75μl

Analyse them on a 1% agarosegel
PCR conditions:
denaturation: 10min
Cycle 25×
denaturtaion: 1min
annealing: 1min (gradient for 60, 65 and 70 degrees)
extension: 1min
Final extension: 10min
Store infinite

Making cloning strategy with biobrick parts


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