IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 31: | Line 31: | ||
* Plastic ware used throughout to prevent alteration of iron concentration | * Plastic ware used throughout to prevent alteration of iron concentration | ||
* pH paper used to test iron stock to be around pH7 | * pH paper used to test iron stock to be around pH7 | ||
<html><br> | |||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
|+Protocol for Varying Iron Concentration in Medium | |+Protocol for Varying Iron Concentration in Medium | ||
Line 48: | Line 47: | ||
|- | |- | ||
|} | |} | ||
<br></br></html> | |||
* Initial OD Reading 30min after addition of Kanamycin and right before incubation = 0.197 | * Initial OD Reading 30min after addition of Kanamycin and right before incubation = 0.197 |
Revision as of 04:47, 7 August 2008
Magnetic Bacteria | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Getting Promoter Plasmid and Iron Assay with Fur- MutantsPromoter Plasmid
Iron Assay with Fur- Mutants
<html><br> {| border="1" cellpadding="2" |+Protocol for Varying Iron Concentration in Medium |- ! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20 |- ! Vol. of Fe stock/ml | 0 || 0.01 || 0.02 || 0.03 || 0.04 || 0.05 |- ! Vol. of SDW/ml | 0.50 || 0.49 || 0.48 || 0.47 || 0.46 || 0.45 |- ! Vol. of fur- cells in M9/ml | 9.5 || 9.5 || 9.5 || 9.5 || 9.5 || 9.5 |- |} <br></br></html>
|